Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Phytochemical screening and hepatoprotective effect of Alhagi maurorum Boiss (Leguminosae) against paracetamol-induced hepatotoxicity in rabbits

Jalil Ur Rehman¹, Naveed Akhtar², Muhammad Younus Khan², Khalil Ahmad¹, Mukhtiar Ahmad¹, Sabira Sultana¹, Hafiz Muhammad Asif²

¹University College of Conventional Medicine, Faculty of Pharmacy & Alternative Medicine, The Islamia University of Bahawalpur; ²Department of Pharmacy, Faculty of Pharmacy & Alternative Medicine, The Islamia University of Bahawalpur; ³Department of Eastern Medicine & Surgery, Faculty of Medical & Health Sciences, The University of Poonch, Rawalakot, AJ& K, Pakistan.

For correspondence:- Hafiz Asif Email: doctor.asif101@gmail.com Tel:+923346911256

Received: 6 October 2014 Accepted: 29 April 2015 Published: 29 June 2015

Citation: Rehman JU, Akhtar N, Khan MY, Ahmad K, Ahmad M, Sultana S, et al. Phytochemical screening and hepatoprotective effect of Alhagi maurorum Boiss (Leguminosae) against paracetamol-induced hepatotoxicity in rabbits. Trop J Pharm Res 2015; 14(6):1029-1034 doi: 10.4314/tjpr.v14i6.13

© 2015 The authors.
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Abstract

Purpose: To evaluate the hepatoprotective activity of aqueous-ethanol (30:70 %) extract of Alhagi maurorum Boiss. (Leguminosae) whole plant against paracetamol-induced liver injury in experimental rabbits.

Methods: Aqueous-ethanol extract of Alhagi maurorum at doses of 250 mg/kg and 500 mg/kg body weight, p.o., was administered for 7 days in paracetamol (2 gm/kg, s.c.) intoxicated rabbits and compared with silymarine (50 mg/kg, p.o.)-treated rabbits. Biochemical parameters, alkaline phosphatase (ALP), serum glutamic oxalacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT) and total bilirubin (TB) levels were recorded to investigate the degree of improvement in the conditions of the rabbits. The liver was removed, washed with normal saline and preserved in 10 % formalin and used in histopathological studies of hepatic architecture by microscopy. Phytochemical screening of the extract was also carried out.

Results: The levels of biochemical parameters were increased in paracetamol intoxicated rabbits when compared with the normal group. The extract, at doses of 250 and 500 mg/kg, exhibited significant (p < 0.001) reduction in biochemical parameters (ALP, SGOT, SGPT and TB). Hepatoprotective activity was also confirmed by histopathological findings. Furthermore, the phytochemical profile of the extract revealed the presence of tannins, alkaloids, saponins and flavonoids.

Conclusion: These results suggest that Alhagi maurorum extract possesses significant hepatoprotective effect against paracetamol-induced hepatotoxicity and this may be due to the presence of flavonoids and tannins.

Keywords: Alhagi maurorum, Hepatoprotective, Paracetamol, Silymarin, Histopathology